Coding

Part:BBa_K1676109:Design

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)


Mutant 6 of Lactate Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers were designed to contain the start and the stop codons so that no mutations would occur on them.


Source

Random mutagenesis via error prone PCR was carried out on the gene.

References